Vesicular stomatitis Indiana virus | |
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TEM micrograph of VSV virions. | |
Virus classification | |
Group: | Group V ((-)ssRNA) |
Order: | Mononegavirales |
Family: | Rhabdoviridae |
Genus: | Vesiculovirus |
Type species | |
Vesicular stomatitis Indiana virus |
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Species | |
Carajas virus |
Vesicular stomatitis Indiana virus (VSIV) (often still referred to as VSV) is a virus in the family Rhabdoviridae; the well-known Rabies virus belongs to the same family. VSIV can infect insects, cattle, horses and pigs. It has particular importance to farmers in certain regions of the world where it can infect cattle. This is because its clinical presentation is identical to the very important Foot and Mouth Disease virus.[1]
The virus is zoonotic and leads to a flu-like illness in infected humans.
It is also a common laboratory virus used to study the properties of viruses in the family Rhabdoviridae, as well as to study viral evolution.[2]
Contents |
VSIV is an arbovirus: natural VSIV infections encompass two steps, cytolytic infections of mammalian hosts and transmission by insects. In insects, infections are non-cytolytic persistent.
Vesicular stomatitis Indiana virus (VSIV) is the prototypic member of the genus Vesiculovirus of the family Rhabdoviridae. The genome of the virus is a single molecule of negative-sense RNA that encodes five major proteins: G protein (G), large protein (L), phosphoprotein, matrix protein (M) and nucleoprotein.
The VSIV G protein enables viral entry. It mediates virus attachment to the host cell, where it is endocytosed. It then mediates fusion of the viral envelope with the endosomal membrane. VSIV enters the cell through partially clathrin coated vesicles; virus-containing vesicles contain more clathrin and clathrin adaptor than conventional vesicles. Virus-containing vesicles recruit components of the actin machinery for their interaction thus inducing its own uptake.
The VSIV L protein is encoded by half the genome, and combines with the phosphoprotein to catalyze replication of the mRNA.
The VSIV M protein is encoded by an mRNA that is 831 nucleotides long and translates to a 229 amino acid-protein. The predicted M protein sequence does not contain any long hydrophobic or nonpolar domains that might promote membrane association. The protein is rich in basic amino acids and contains a highly basic amino terminal domain.
After infection, the VSIV G gene is expressed and is commonly studied as a model for N-linked glycosylation in the endoplasmic reticulum. It is translated into the rough ER where the Glc3-Man9-GlcNac2 oligosaccharide is added by a dolichol-containing protein, to an NXS motif on VSIV G. Sugars are removed gradually as the protein travels to the Golgi apparatus, and it becomes resistant to endoglycosidase H.[3]
VSIV G does not follow the same path as most vesicles because transport of the G protein from the endoplasmic reticulum to the plasma membrane is interrupted by incubation at 15°C. Under this condition, the molecules accumulate in both the endoplasmic reticulum (ER) and a subcellular vesicle fraction of low density called the lipid-rich vesicle fraction. The material in the lipid-rich vesicle fraction appears to be a post-ER intermediate in the transport process to the plasma membrane (PM). When synthesized in polarized epithelial cells, the envelope glycoproteins hemagglutinin of VSIV G are targeted to the apical and basolateral plasma membranes. VSVG is also a common coat protein for lentiviral vector expression systems used to introduce genetic material into in vitro systems or animal models, mainly because of its extremely broad tropism.
The main sign in animals is oral disease appearing as mucosal vesicles and ulcers in the mouth, but also on the udder and around the coronary band. Animals may show systemic signs such as anorexia, lethargy and pyrexia. Disease usually resolves within 2 weeks and animals usually recover completely.[1]
Serological testing is most commonly performed with en ELISA or complement fixation and viral isolation can also be attempted.[1]
No specific treatment is available, but some animals may require supportive fluids or antibiotics for secondary infections.[1]
Control relies on biosecurity protocols, quarantine, isolation and disinfection to ensure the viral disease does not enter a country or herd.[1]
VSIV has oncolytic activity, leading to preferential killing of infected tumor cells.[4] Recently, attenuated VSIV with a mutation in its M protein has been found to have oncolytic properties. Research is ongoing, and has shown VSV to reduce tumor size and spread in melanoma, lung cancer, colon cancer and certain brain tumors in laboratory models of cancer.[5]
The VSIV G protein is commonly used in biomedical research to pseudotype retroviral and lentiviral vectors, conveying the ability to transduce a broad range of mammalian cell types with genes of interest[6].
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